Fast, precise, and cost-effective targeted molecular karyotyping
BACs are Bacterial Artificial Chromosomes, large cloned sequences of human DNA typically 170,000 bases long. BAC probes have long been used for FISH. The BACs-on-Beads? technology uses probes generated from selected BACs immobilized onto Luminex? xMAP? encoded beads. The resulting bead sets are used to assay chromosomal gains and losses from minute sample amounts with high throughput. BACs-on-Beads analysis comprises hybridization of DNA sample sequences to BAC-derived probes representing the identified target regions. Samples are enzymatically labeled with biotin, and after hybridization in a 96-well plate a fluorescent streptavidin-phycoerythrin reporter is bound to the biotin label. Male and female reference DNA is also hybridized and the fluorescence ratio between each sample and reference DNA are compared to provide a clear display of any copy number changes in the targeted regions. Sample labeling is done enzymatically, which provides significant amplification of samples down to 100-200 ng.
For research use only. Not for use in diagnostic procedures.