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Operetta CLS 高內涵系統

Operetta CLS? 系統幫助您探索常規實驗與復雜創新實驗中的生物學信息。通過一系列創新技術的有機結合,Operetta CLS? 同時滿足精細細胞細節成像對高速、高靈敏度、高分辨率檢測多方面的需求。同時,憑借功能強大的Harmony 4.5版軟件,Operetta CLS? 可幫助您發現并定量細微的細胞表型改變。

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Operetta CLS? 系統同時兼具高速、高靈敏度的數據采集能力,以及廣受贊譽的功能強大,界面友好的數據分析解讀功能。為了進一步助力您的研究工作,我們提供了從全套高內涵篩選的解決方案,從高內涵儀器到成像使用標準多孔板到自動化液體工作站以及后續豐富的信息學數據處理解決方案。Perkinelmer為你提供一站式的無縫連接服務,幫助您搭建全面專業高內涵篩選平臺。完善的平臺搭建服務與界面友好且功能強大的高內涵分析軟件harmony ,幫您掃除高內涵篩選路上的后顧之憂。與您一路同行,快速高效的開始您的高內涵篩選工作。

多項專為高內涵篩選研發的先進技術的有機組合

Operetta CLS高內涵篩選系統以全新設計的高靈敏的光路為核心,確保最高效的樣品信息的激發和檢測。

  • 首開先河的高能、長壽命8色高內涵固態LED光源提供最優化、最可靠地熒光信號采集和數據采集。
  • 專利全自動水鏡系統同時滿足了細胞圖像采集在高分辨率、高速以及光毒性最小的需求。
  • 轉盤共聚焦提供了快速、低光毒性的共聚焦成像,在獲得共聚焦質量圖像的同時,提供了活細胞在線實時檢測以及三維掃描檢測功能。
  • 大靶免sCMOS相機提供了低噪聲、寬動態范圍、高分辨率的圖像檢測,為低光毒性、高靈敏度、定量檢測提供了絕佳的檢測方案。
  • 用戶友好的編碼型濾光片,提供了靈活、特異性高、且易于操作的成像體驗。
  • 簡單且功能強大的Harmony 4.5高內涵軟件成像分析軟件提供了符合生物學使用習慣、邏輯清晰、功能強大的實驗設置、圖像采集、圖像分析以及數據存儲和解讀。

為您實驗室量身定做的高內涵解決方案:

Operetta CLS? 滿足您實驗中的多種實驗需求。您可根據您的實驗需求定制不同模塊的高內涵系統。推薦配置包括:

Operetta CLS? Quattro

  • 四色LED以及寬場熒光,Operetta CLS基礎配置滿足您實驗室日常實驗所需要的靈敏度和分辨率,同時為您提供了保留了豐富的升級空間。

Operetta CLS? FLEX

  • 八色LED,共聚焦及寬場熒光成像,FLEX 配置提供全面的、靈活的、特異性極高的熒光激發、熒光檢測配置,同時該型號也提供更加高端的性能升級空間。

Operetta CLS? LIVE

  • 在FLEX型號基礎上,配置活細胞氣體控制與溫度控制以及低光毒性、高分辨率、高熒光亮度的水鏡頭系統,該系統是低光毒性、高靈敏度的活細胞成像實驗的理想選擇。

規格

21 CFR Part 11兼容 No
自動化兼容 Yes
檢測方法 Transmission, Fluorescence
高度 45.0 cm
顯像模式 High Content
接口 Ethernet
長度 98.0 cm
光源 LED
便攜式 No
產品品牌名稱 Operetta CLS
保修 Yes
寬度 66.0 cm
資源
  • 所有

網絡研討會

Image-Based Screening for Precision Medicine and Immunotherapy

Discover how image-based screening can be performed directly in primary material, enabling more clinically translatable drug screening, and ultimately, functional precision medicine.

網絡研討會
HCS for Predictive Toxicology and in vitro Pathology Using Simple 3D Microtissues

This webinar will describe the integration of biology and engineering to devise simple, high-throughput 3D human microtissues as predictive biology platforms that reflect human physiology and disease.

網絡研討會
Face-to-Face with Cancer: The Power of High-Content Imaging and Analytics to Illuminate Cell Population Dynamics

To advance our biological understanding of cancer and improve treatment efficacy, we are utilizing quantitative high-content imaging to illuminate the dynamic interactions between cancer cells and their microenvironment.

網絡研討會
Moving from 2D to 3D cell models: Overcoming challenges to achieving high-quality high-content results

Over recent years, more biologists have started using 3D cellular models, which provide cells with something more akin to their natural microenvironment. However, they present a number of challenges to be overcome.

網絡研討會
Single Cell Phenotypic Analysis Using High-Content Imaging

In this webcast, Dr Chris Bakal, from the Institute of Cancer Research, London, will describe the power of HCA to quantify cellular single cell phenotypes and to measure cell-to-cell variability and phenotypic heterogeneity.

網絡研討會

產品手冊

3D Cell Culture Solutions Brochure

Download our brochure to learn how our solutions help you to grow, detect, and analyze 3D cells.

PDF 4 MB
HCS Operetta CLS 應用案例

高內涵應用案例

PDF 6 MB
Operetta CLS

高內涵成像篩選系統Operetta CLS

PDF 1 MB
藥物篩選完全解決方案

從高通量到高內涵藥物篩選完全解決方案

PDF 1 MB

小冊子

Adding Depth: 3-D Cell Culture

Download this booklet from The Scientist and PerkinElmer to learn about how the third dimension affects cell behavior, the similarities and differences between 2-D and 3-D culture, common 3-D culture models, and how to image and analyze 3-D culture models.

PDF 5 MB
Cellular Analysis in 3D

While 3D cell culture provides unprecedented opportunities for both increased physiological relevance and analysis using a high-content approach, it is also more complex than traditional 2D cell culture. This booklet, from Biocompare and PerkinElmer, will unravel some of the complexities often encountered when using 3D cell models for drug discovery and provide insights and solutions that will streamline workflows and facilitate the development of effective therapeutics. Topics covered include: Reagents and instruments for growing, detecting, and analyzing 3D cell models; 3D culture methodologies; the value of high-content screening with 3D cell models and how to improve image acquisition and image analysis with high-content assays.

PDF 2 MB
High-Content Analysis: Advancing Your Knowledge to Help Combat Infectious Disease

Infectious diseases remain a major burden to human health. The increased globalization of modern society that facilitates the spread of infectious diseases, and phenomena such as anti-microbial resistance, underscore the importance of the development of new preventative and therapeutic approaches.

Download this booklet to learn how high-content imaging and analysis enable high-throughput functional and phenotypic assays that can be adapted to a wide range of pathogens; read a series of Featured Publication Notes describing the contribution of high-content analysis in the study of diseases such as ebola, zika, tuberculosis, listeria and malaria and find examples of studies in which a high-content approach has been used in parasitic, viral and bacterial disease research.

PDF 4 MB

應用文獻

Analyzing ERK Signal Transduction in Live Cells Using a FRET-Based Biosensor

Extracellular signal-regulated kinase (ERK) is a key component in the regulation of embryogenesis, cell differentiation, cell proliferation, and cell death. The ERK signaling pathway is altered in various cancer types and is frequently investigated as a target for therapeutic intervention. This application note describes how a live cell FRET assay to study ERK signaling was performed on the Operetta CLS? high-content analysis system. The optimized design of the FRET-based biosensor, the high-quality imaging of the Operetta CLS system and the easy-to-use image analysis tools of the Harmony? software contribute to the robustness of the high-content assay.

PDF 1 MB
Automated Single Cell Tracking using the Operetta System: Analyzing Chemokinesis of Cancer Cells

In this application note, we describe a high-content screening application for analyzing the migration of non-small cell lung cancer cells in a live cell assay. Using the Operetta? high-content imaging system and digital phase contrast imaging, we tracked migrating cancer cells using automated single cell tracking in the Harmony? high-content imaging and analysis software.

PDF 1 MB
Distinguishing Cell Types by Phenotypic Profiling of the Nucleus

The promise of high-content screening is the acceleration of discovery by extracting as much relevant information as possible from cells. Nevertheless, a large percentage of high-content screens analyze only a small number of image-based properties. As a result, valuable information from precious cells and disease models is not utilized. As nearly all screening approaches require a nuclear counterstain such as Hoechst to facilitate segmentation, phenotypic profiling of the nuclei can offer new and additional perspectives on assays at no extra cost.

PDF 1 MB
Phenotypic Analysis of Hypertrophy in Human iPSC-Derived Cardiomyocytes

Learn how a phenotypic screening assay to study time-dependent effects of endothelin-1-induced hypertrophy was set up using human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes. Learn how: The Opera Phenix system has been applied in the field of neurodegenerative diseases. In this assay, the Opera Phenix system is 4 times faster than the previous Opera? system. Primary neuron morphology is analyzed in a straightforward approach using Harmony software. Careful assay optimization can increase throughput, and minimize the data burden, without compromising assay performance.

PDF 1 MB

技術說明

3D Volumetric Analysis of Luminal Spaces Inside Cysts or Organoids

High-content assays using 3D objects such as cysts or organoids can be challenging from the perspectives of both image acquisition and image analysis. In this technical note we describe how to image and analyze epithelial Madin-Darby canine kidney (MDCK) cysts in 3D on the Operetta CLS? high-content analysis system.

We address:

  • How to reduce the loss of image resolution as you image deeper into the sample
  • How to enable 3D segmentation and analysis for your 3D high-content assays
  • How to increase throughput for 3D imaging through shorter exposure times
  • How to eliminate long and tedious data transfer steps

PDF 1 MB
3D Volumetric and Zonal Analysis of Solid Spheroids

Multicellular 3D “oids” (tumoroids, spheroids, organoids) have the potential to better predict the effects of drug candidates during preclinical screening. However, compared to 2D cell monolayers, assays using 3D model systems are more challenging.

In this technical note we describe how to image and analyze solid spheroids in 3D using the Opera Phenixand OperettaCLS high-content screening systems and Harmony® 4.8 imaging and analysis software.

We address:

  • How to reduce image acquisition time and data volume
  • How to analyse fully and partially imaged spheroids in 3D
  • How to define zones and quantify spatial differences within spheroids
  • PDF 1 MB
    Clearing Strategies for 3D Spheroids

    In drug discovery programs, multicellular spheroids have emerged as powerful tools to bridge the gap between in vitro cell culture models and in vivo tissues. However, one of the greatest challenges in higher throughput 3D imaging is the acquisition of images of solid spheroids, owing to the reduced light penetration.

    One solution is to use optical clearing techniques, which can enhance the imaging depth in spheroids by removing lipid and protein molecules.

    In this technical note, we compare different optical clearing strategies for 3D spheroids and quantify the clearing effectiveness and alterations in spheroid morphology, and demonstrate how to increase imaging depth in 3D spheroids by a factor of four.

    PDF 1 MB
    How to Perform Successful Long Term Live Cell Imaging in a High-Content Analysis System

    Download our technical note to find out how you can overcome some of the challenges associated with long-term live cell imaging. Learn how you can perform successful five-day live cell imaging on Operetta CLS? and Opera Phenix? high-content systems, avoid phototoxicity with gentle digital phase contrast imaging, and analyze cell growth and morphology on a single cell level without fluorescence staining.

    PDF 3 MB
    The Benefits of Automated Water Immersion Lenses for High-Content Screening

    Balancing the key factors in HCS imaging - sensitivity, resolution and speed - can be challenging since they cannot be optimized independently: changing one impacts the others. Nevertheless, there is a way to overcome some of the obstacles and here we explain why the choice of the objective lens is critical.

    PDF 1 MB

    操作手冊

    Five Top Tips for a Successful High-Content Screening Assay with a 3D Cell Model System

    Whether you’re familiar with high-content screening, or a newcomer, you’ll need the right tools and strategies to overcome the challenges of using complex 3D cell models in such an assay. For example, growing consistent, reproducible 3D cultures can be problematic and imaging large, thick cell samples can be challenging, while managing the huge volumes of data generated is perhaps the most demanding aspect of all. In this article, we provide our top tips for running a successful high-content screening assay using a 3D cell model. Learn how you can: Generate uniform 3D cell models, Get the best quality images, Minimize imaging time and volume of data, Get deeper insights from your 3D cell model and Avoid unnecessary data transfer steps.

    PDF 1 MB

    文章

    5 Top Tips for a Successful High-content Screening Assay with a 3D Cell Model System

    Whether you’re familiar with high-content screening and are looking to exploit the increased physiological relevance of complex 3D cell models, or you want to take your analysis of 3D cell models to the next level, migrating from simple plate-reader assays to a high-content approach, you’ll need the right tools and strategies to overcome the challenges these models present.

    PDF 1 MB

    案例分析

    A Workflow to Characterize and Benchmark Human Induced Pluripotent Stem Cells

    Human induced pluripotent stem cells (iPSCs) offer tremendous opportunities for disease modeling and discovery of novel therapeutics. The UK-based Human Induced Pluripotent Stem Cell Initiative (HipSci) aims to advance iPSC technology and pave the way to discovering how genomic variation impacts cellular phenotype by offering the scientific community access to a vast panel of cell lines with thorough characterization and data analysis tools. This case study details a phenotypic screen used to characterize human iPSCs on diverse extracellular matrix substrates, and a method for the capture of specific phenotypes emerging upon cell-to-cell contact.

    PDF 1 MB
    High-Content Analysis of Drug-Induced Oligodendrocyte Differentiation Promoting Remyelination in Multiple Sclerosis

    One of the greatest challenges in multiple sclerosis (MS) therapy is the halting or reversal of the failure of remyelination in the brain in order to reverse disabilities in MS patients. This case study highlights the recent work of Dr. Paul Tesar and colleagues at the Case Western Reserve University School of Medicine, which could potentially lead to such novel treatments, as it aims to control the function of stem cells in the body and thereby to help the body repair itself.

    PDF 3 MB

    白皮書

    High Content Screening in Three Dimensions

    Researchers are increasingly looking to 3D cell cultures, microtissues, and organoids to bridge the gap between 2D cell cultures and in vivo animal models. This whitepaper documents a streamlined procedure for getting the most information, as quickly as possible, using solutions from PerkinElmer.

    PDF 1 MB
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